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pTK5

HSV I thymidine kinase

Catalog number RDB01027
Resource name pTK5
Alternative name HSV I thymidine kinase
Clone info. Herpes simplex virus type I thymidine kinase
Comment At least covering 46589..47442 and 47550..47719 of GU734771.1. Reference for insert sequence, Wigler, M., Cell, 14 (3): 725-731 (1978).
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4,361 bp
Selectable markers Amp^r
Gene/insert name HSV thymidine Kinase Genomic DNA
Depositor|Developer Soeda, Eiichi |
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External Database
HSV thymidine Kinase

          Reference sequence
            

          Distribution information

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          RDB01027 pTK5 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01027.gif

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0010e (English text)
          Featured content Dnaconda's recommendation EXR0010j (Japanese text)
          Featured content Suicide gene therapy (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12799_A4F2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBR322_BamHI_F
          Sequence file: RDB12799_A4F2a.seq check
          >01027_12799_A4F2_pBR322_BamHI_F_B10_04.ab1
              1 GAAGGTTCTG TGGGATCAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA
             61 GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC
            121 GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC
            181 CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT
            241 CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG
            301 CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG
            361 ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA
            421 GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA
            481 GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG GAGTGGCGCA
            541 GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT
            601 ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA
            661 ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG CATATTAAGG
            721 TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAC AGCGTCAACA
            781 GCGTGCCGCA GATCTTGGTG GCGTGAAACT CCCGCACCTC TTCGGCAAGC GCCTTGTAGA
            841 AGCGCGTATG GCTTCGTACC CCTGCCATCA ACACGCGTCT GCGTTCGACC AGGCTGCGCG
            901 TTCTCGCGGC CATAGCAACC GACGTACGGC GTTGCGCCCT CGCCGGCAGC AAGAAGCCAC
            961 GGAAGTCCGC CTGGAGCAGA AAATGCCCAC GCTACTGCGG GTTTATATAG ACGGTCCCTC
           1021 ACGGGATGGG GGAAAACCCA CCCACCACGC AACTGCTGGG TGGGCCCTGG GGCTCGCGCG
           1081 ACGATATCGT CTACGTTACC CGAGCCGAAT GACTTACTGC CAAGTGCCTG GGGGCATCTC
          1141 GGACATCGCG ACATTCTTAT ACAATCCTC
          //
          Primer: pBR322_BamHI_R
          Sequence file: RDB12799_A4F2b.seq check
          >01027_12799_A4F2_pBR322_BamHI_R_C10_07.ab1
              1 GCGTTGAGAA ACACAGAATC CGCGGCACAG CCCGCGGCCC CGCGGGCGGC CAACCCGGCA
             61 AGCGCGCGCG AGTGGGCCAA AAAGCCTAGC AGGTCGGAGA GGCAGACCGC GCCGTTTGCG
            121 TGGGCGGCGT TCACGAAAGC AAAACCCGAC GTCGCGAGCA GCCCCGTTAG GCGCCAGAAG
            181 AGAGGGGGGC GCGGGCCCTG CTCGGCGCCC GCGTCCCCCG AGAAAAACTC CGCGTATGCC
            241 CGCGACAGGA ACTGGGCGTA GTTCGTGCCC TCCTCCGGGT AGCCGCCCAC GCGGCGGAGG
            301 GCGTCCAGCG CGGAGCCGTT GTCGGCCCGC GTCAGGGACC CTAGGACAAA GACCCGATAC
            361 CGGGGGCCGC CCGGGGGCCC GGGAAGAGCC CCCGGGGGGT TTTCGTCCGC GGGGTCCCCG
            421 ACCCGATCTA GCGTCTGGCC CGCGGGGACC ACCATCACTT CCACCGGAGG GCTGTCGTGC
            481 ATGGATATCA CGAGCCCCAT GAATTCCCGC CCGTAGCGCG CGCGCACCAG CGCGGCATCG
            541 CACCCGAGCA CCAGCTCCCC CGTCGTCCAG ATGCCCACGG GCCACGTCGA GGCCGACGGG
            601 GAGAAATACA CGTACCTACC TGGGGATCTC AACAGGCCCC GGGTGGCCAA CCAGGTCGTG
            661 GACGCGTTGT GCAGGTGCGT GATGTCCAGC TCCGTCGTCG GGTGCCGCCG GGCCCCAACC
            721 GGCGGTCGGG GGGGCGGTGT ATCACGCGGC CCCGCTTGGG TGGCTCGCCC GTCGCCACGT
            781 TTGTCTCCCC GCGGGGAACG TCAGGGCCCT CGGGTTCAGG GACGGCCGAA AACGTTACCC
            841 AGGCCCGGGA ACGGCAACTA CACGGAAGCG ACTGGATTTG TACAATAGAC CCCTTAAGGG
            901 GGCCGACCGA AGGGGGGGAG GGCTGGGCCG GTTCGGCTCG ACCGTGGTCG GGGGCGGGCA
            961 GGCTCGCCGT TTCCGGGGGC CCGGGCTGAA GCAGCGTAAG GTCCTTCGGG CACTGTAAAA
          1021 GGAA
          //
          Primer: HSV_TKpA_R
          Sequence file: RDB12799_A4F2c.seq check
          >01027_12799_A4F2_HSV_TKpA_R_C10_07.ab1
              1 ACGGGGTTCT TCCGGGTATT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCATCTCCCG
             61 GGCAAACGTG CGCGTCTCAG GTCGCAGATC GTCGGTATGG AGCCGGGGGT GGTGACGTGG
            121 GTCTGGACCA TCCCGGAGGT AAGTTGCAGC AGGGCGTCCC GGCAGCCGGC GGGCGATTGG
            181 TCGTAATCCA GGATAAAGAC GTGCATGGGA CGGAGGCGTT TGGCCAAGAC GTCCAAGGCC
            241 CAGGCAAACA CGTTGTACAG GTCGCCGTTG GGGGCCAGCA ACTCGGGGGC CCGAAACAGG
            301 GTAAATAACG TGTCCCCGAT ATGGGGTCGT GGGCCCGCGT TGCTCTGGGG CTCGGCACCC
            361 TGGGGCGGCA CGGCCGTCCC CGAAAGCTGT CCCCAATCCT CCCGCCACGA CCCGCCGCCC
            421 TGCAGATACC GCACCGTATT GGCAAGCAGC CCGTAAACGC GGCGAATCGC GGCCAGCATA
            481 GCCAGGTCAA GCCGCTCGCC GGGGCGCTGG CGTTTGGCCA GGCGGTCGAT GTGTCTGTCC
            541 TCCGGAAGGG CCCCCAACAC GATGTTTGTG CCGGGCAAGG TCGGCGGGAT GAGGGCCACG
            601 AACGCCAGCA CGGCCTGGGG GGTCATGCTG CCCATAAGGT ATCGCGCGGC CGGGTAGCAC
            661 AGGAGGGCGG CGATGGGATG GCGGTCGAAG ATGAGGGTGA GGGCCGGGGG CGGGGCATGT
            721 GAGCTCCCAG CCTCCCCCCC GATATGAGGA GCCAGAACGG CGTCGGTCAC GGCATAAGGC
            781 ATGCCCATTG TTATCTGGGC GCTTGTCATT ACCACCGCCG CGTCCCCGGC CGATATCTCA
            841 CCCTGGTCGA GGCGGTGTTG TGTGGTGTAG ATGTTCGCGA TTGTCTCGGA AGCCCCCCAG
            901 CACCTGCCAG TAAGTCATCG GCTCGGGGTA CGTAGACGAT ATCGTCGCGC GAACCCAGGG
            961 CCACCAGCAG TTGCGTGGTG GTGGTTTTCC CCATCCCGTG AGGACCGTTC TATATAAACC
           1021 CGCAGTTAGC GTGGGCAATT TTTCTGCTTC CAGGCGGACT TTCCCGTGGC CTTCCTTGCT
           1081 TGCCCGGGCC GAGGGGGCGC CAAACGGTCG TACGATCCGA TTGCCTTATG GTCGGCCGAG
          1141 GAAACCGCCT GGCCAGAGCC CCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Wigler, M., Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell, 14 (3): 725-731 (1978). PMID 210957.
          user_report Kurayoshi, K., Cancer cell specific cytotoxic gene expression mediated by ARF tumor suppressor promoter constructs. Biochem. Biophys. Res. Commun., 450 (1): 240-246 (2014). PMID 24893334.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl