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pBKV525

Human BK virus 525

Catalog number RDB01019
Resource name pBKV525
Alternative name Human BK virus 525
Clone info. Human DNA virus BKV 525
Vector backbone pBR325 (Plasmid)
Size of vector backbone 6.0 kb
Selectable markers Amp^r
Gene/insert name Hum_Polyoma BKV transforming gene Genomic DNA
Depositor|Developer Soeda, Eiichi |
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External Database
Hum_Polyoma BKV transforming gene

            Reference sequence
              

            Distribution information

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            RDB01019 pBKV525 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (gif) RDB01019.gif

            Original reference

            original Watanabe, S., DNA rearrangement affecting expression of the BK virus transforming gene. J. Virol., 51, 1-6 (1984). PMID 6328028.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12317_A3Kj.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12317_A3Kja.seq check
            >01019_12317_A3Kj_Amp_R_G10_19.ab1
                1 CTCCATTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
               61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
              121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
              181 CGAGGCCCTT TCGTCTTCAA GAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTTTTC
              241 TCATTAAGGG AAGATTTCCC CAGGCAGCTC TTTCAAGGCC TAAAAGGTCC ATGAGCTCCA
              301 TGGATTCTTC CCTGTTAAGA ACTTTATCCA TTTTTGCAAA AATTGCAAAA GAATAGGGAT
              361 TTCCCCAAAT AGTTTTGCTA GGCCTCAGAA AAAGCCTCCA CACCCTTACT ACTTGAGAGA
              421 AAGGGTGGAG GCAGAGGCGG CCTCGGCCTC TTATATATTA TAAAAAAAAA GGCCACAGGG
              481 AGGAGCTGCT TACCCATGGA ATGCAGCCAA ACCATGACCT CAGGAAGGAA AGTGCATGAC
              541 TGGGCAGCCA GCCATGACCT CAGGAAGGAA AGTGCATGAC TGGGCAGCCA GCCAGTGGCA
              601 GTTAATAGTT AAACTGGACA AAGGAAAGTG CATGACTGGG CAGCCAGCCA TGACCTCAGG
              661 AAGGAAAGTG CATGACTGGG CAGCCAGCCA GTGGCAGTTA ATAGTTAAAC TGGACAAAGG
              721 CCATGGTTCT GCGCCAGCTG TCACGACAAG CTTTAATGCG GTAGTTTATC ACAGTTAAAT
              781 TGCTAACGCA GTCAGGCACC GTGTATGAAA TCTAACAATG CGCTCATCGT CATCCTCGGC
              841 ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC CGGTACTGCC GGGCCTCTTG
              901 CGGGATATCG TCCATTCCGA CAGCATCGCC AGTCACTATG GCGTGCTGCT AGCGCTATAT
              961 GCGTTGATGC AATTTCTATG CGCACCCGTT CTCGGAGCAC TGTCCGACCG CTTTGGGCCG
             1021 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC
             1081 CACACCCGTC CCTGTGATCT CTACGCCGGG ACGCATCGTG GTCGCCATCA CCGGCGCCAC
            1141 AGGTGCGATG CCTGCGCTTA TTATCGCCGA ACCTTCACCG AAATGGGGGA GAT
            //
            Primer:
            Sequence file: RDB12317_A3Kjb.seq check
            >01019_12317_A3Kj_pBR322_BamHI_R_D08_11.ab1
                1 TGGATTTAGG GCCCCCATGG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC CCAATCGAGC
               61 AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT
              121 TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG
              181 ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC
              241 AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA
              301 CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTGTCGTG ACAGCTGGCG
              361 CAGAACCATG GCCTTTGTCC AGTTTAACTA TTAACTGCCA CTGGCTGGCT GCCCAGTCAT
              421 GCACTTTCCT TCCTGAGGTC ATGGCTGGCT GCCCAGTCAT GCACTTTCCT TTGTCCAGTT
              481 TAACTATTAA CTGCCACTGG CTGGCTGCCC AGTCATGCAC TTTCCTTCCT GAGGTCATGG
              541 CTGGCTGCCC AGTCATGCAC TTTCCTTCCT GAGGTCATGG TTTGGCTGCA TTCCATGGGT
              601 AAGCAGCTCC TCCCTGTGGC CTTTTTTTTT ATAATATATA AGAGGCCGAG GCCGCCTCTG
              661 CCTCCACCCT TTCTCTCAAG TAGTAAGGGT GTGGAGGCTT TTTCTGAGGC CTAGCAAAAC
              721 TATTTGGGGA AATCCCTATT CTTTTGCAAT TTTTGCAAAA ATGGATAAAG TTCTTAACAG
              781 GGGAAGAATC CATGGAGCTC ATGGACCTTT TAGGCCCTTT GAAAGAGCTG CCTGGGGAAA
              841 TCTTCCCTTA ATGAGAAAAA GCTTATCGAT GATAAGCTGT TCAAACATGA GAAATTCTTG
              901 AAGACGAAAG GGCCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG
              961 TTTCTTAAGA CGTCAGGTGG CACTTTTTCG GGAAATGTGC GCGGAACCCC CTATTTGTTT
             1021 ATTTTTCTAA ATACATTTCA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
             1081 TTCATAATAT TGAAAGGAAG AAGTATGAGT ATTTCAACAT TTCCGTGTCG CCTTATTCCC
             1141 TTTTTTGCGG CAATTTGCCT TCCTGATTTT GCTCCACCCA AGAAACGCCT GGGGTAAAGC
            1201 TAAAAGAAAT GGCCTCTGTG CAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Watanabe, S., DNA rearrangement affecting expression of the BK virus transforming gene. J. Virol., 51, 1-6 (1984). PMID 6328028.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl