Resource data sheet
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pSTE33

Bacillus stearothermophilus shuttle vector

Catalog number RDB00920
Resource name pSTE33
Clone info. Bacillus stearothermophilus shuttle vector.
Vector backbone pSTE33 (Plasmid)
Size of vector backbone 5,650 bp
Selectable markers Amp^r (E. coli), Kan^r (B. stearothermophilus)
Growth remarks 55 degC for Bacillus stearothermophilus ( LB+Kan 10 micro g/mL)
Reference of nucleotide sequence D29979 (DDBJ accession number)
Depositor Nakamoto, Shinya | Narumi, Issay |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Narumi, I., Biotechnol. letters, 15, 815-820 (1993)).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB00920 pSTE33 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Narumi, I., Construction of a new shuttle vector pSTE33 and its stabilities in Bacillus stearothermophilus, Baciluss subtilis, and Escherichia coli. Biotechnol. letters, 15, 815-820 (1993).

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0011j (Japanese text)
Featured content Bacteria host strains (en) (English text)
Featured content Bacteria host strains (ja) (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Narumi, I., Construction of a new shuttle vector pSTE33 and its stabilities in Bacillus stearothermophilus, Baciluss subtilis, and Escherichia coli. Biotechnol. letters, 15, 815-820 (1993).
user report Carr, S.B., Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 69 (Pt 10): 1123-1126 (2013). PMID 24100563.

2020.01.22

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