Resource data sheet


Bacillus stearothermophilus shuttle vector

Catalog number RDB00920
Resource name pSTE33
Clone info. Bacillus stearothermophilus shuttle vector.
Vector backbone pSTE33 (Plasmid)
Size of vector backbone 5,650 bp
Selectable markers Amp^r (E. coli), Kan^r (B. stearothermophilus)
Gene/insert name vector -
Reference of nucleotide sequence D29979 (DDBJ accession number)
Depositor Nakamoto, Shinya | Narumi, Issay |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Narumi, I., Biotechnol. letters, 15, 815-820 (1993)).
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB00920 pSTE33 DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0011j (Japanese text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)


original Narumi, I., Construction of a new shuttle vector pSTE33 and its stabilities in Bacillus stearothermophilus, Baciluss subtilis, and Escherichia coli. Biotechnol. letters, 15, 815-820 (1993).
user report Carr, S.B., Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 69 (Pt 10): 1123-1126 (2013). PMID 24100563.