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pSTE33 (#RDB00920)

Bacillus stearothermophilus shuttle vector

Clone info. Bacillus stearothermophilus shuttle vector.
Vector backbone pSTE33 (Plasmid)
Size of vector backbone 5,650 bp
Selectable markers Amp^r (E. coli), Kan^r (B. stearothermophilus)
Growth remarks 55 degC for Bacillus stearothermophilus ( LB+Kan 10 micro g/mL)
Depositor|Developer Nakamoto, Shinya | Narumi, Issay |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Narumi, I., Biotechnol. letters, 15, 815-820 (1993)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Narumi, I., Biotechnol. letters, 15, 815-820 (1993))。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB00920 pSTE33 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pSTE33 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB00920).

Reference section:

Narumi, I., Nakayama, N., Makamoto, S., Kimura, T., Yanagisawa, T., Kihara, H., Construction of a new shuttle vector pSTE33 and its stabilities in Bacillus stearothermophilus, Baciluss subtilis, and Escherichia coli. Biotechnol. letters, 15, 815-820 (1993). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Narumi, I., Construction of a new shuttle vector pSTE33 and its stabilities in Bacillus stearothermophilus, Baciluss subtilis, and Escherichia coli. Biotechnol. letters, 15, 815-820 (1993). [link to RRC of NBRP]
user_report Carr, S.B., Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 69 (Pt 10): 1123-1126 (2013). PMID 24100563. [link to RRC of NBRP]