Bacillus stearothermophilus shuttle vector
| Catalog number | RDB00919 |
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| Resource name | pSTE12 |
| Clone info. | Bacillus stearothermophilus shuttle vector |
| Vector backbone | pSTE12 (Plasmid) |
| Size of vector backbone | 5,800 bp |
| Selectable markers | Amp^r (E. coli), Tet^r (B. stearothermophilus) |
| Growth remarks | 55 degC for Bacillus stearothermophilus (LB+Tet). |
| Depositor | Nakamoto, Shinya | Narumi, Issay | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nakayama, N., Biotechnol. letters, 14, 649-652 (1992)). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB00919 | pSTE12 | DNA solution |
Electronic file
| Electronic file | Map RDB00919.gif from Depositor
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| Electronic file | Remarks, protocol and/or map (pdf) RDB00919.pdf from Depositor |
Original reference
| original | Nakayama, N., A new shuttle vector for Bacillus stearothermophilus and Escherichia coli. Biotechnol. letters, 14, 649-652 (1992). |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Bacteria host strains (en) (English text) |
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| Featured content | Bacteria host strains (ja) (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB00919_A6Dmp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: SV40pA3'Element Reverse | Sequence: RDB00919_A6Dma.seq |
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>00919_00919_A6Dm_SV40pA3ElementReverse_A09_03.ab1 1 GTTTTGGGGG GGGCGGACCT ATGGAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 301 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 361 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 421 GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCGACCTGC 481 AGGCATGCAA GCTTGGCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC 541 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA 601 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG 661 ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC 721 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT 781 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC 841 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG 901 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 961 TCAAGTGGCA CTTTTTCGGG GGGAAAATGT GCGCGGGAAA CCCCCTATTG TTTAATTTTT 1021 CTAAATACAT TCAAATAATG TATTCGCTTC ATGAGACAAT TAACCCTTGA TAATGGCTTT 1081 CAATAATAAT ACCTGGTATA AAAGGATCGA TTTGAAAC // |
| Primer: pGEX-R | Sequence: RDB00919_A6Dmb.seq |
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>00919_00919_A6Dm_pGEX_R_B09_06.ab1 1 TCATACGTAC GCGCGAGACG AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT 61 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC 121 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG 181 ATAAATGCTT CAATAATAAT TCCTGTTATA AAAAAAGGAT CAATTTTGAA CTCTCTCCCA 241 AAGTTGATCC CTTAACGATT TAGAAATCCC TTTGAGAATG TTTATATACA TTCAAGGTAA 301 CCAGCCAACT AATGACAATG ATTCCTGAAA AAAGTAATAA CAAATTACTA TACAGATAAG 361 TTGACTGATC AACTTCCATA GGTAACAACC TTTGATCAAG TAAGGGTATG GATAATAAAC 421 CACCTACAAT TGCAATACCT GTTCCCTCTG ATAAAAAGCT GGTAAAGTTA AGCAAACTCA 481 TTCCAGCACC AGCTTCCTGC TGTTTCAAGC TACTTGAAAC AATTGTTGAT ATAACTGTTT 541 TGGTGAACGA AAGCCCACCT AAAACAAATA CGATTATAAT TGTCATGAAC CATGATGTTG 601 TTTCTAAAAG AAAGGAAGCA GTTAAAAAGC TAACAGAAAG AAATGTAACT CCGATGTTTA 661 ACACGTATAA AGGACCTCTT CTATCAACAA GTATCCCACC AATGTAGCCG AAAATAATGA 721 CACTCATTGT TCCAGGGAAA ATAATTACAC TTCCGATTTC GGCAGTACTT AGCTGGTGAA 781 CATCTTTCAT CATATAAGGA ACCATAGAGA CAAACCCTGC TACTGTTCCA AATATAATTC 841 CCCCACAAAG AACTCCAATC ATAAAAGGTA TATTTTTCCC TAATCCGGGA TCAACAAAAG 901 GATCTGTTAC TTTCCTGATA TGTTTTACAA ATATCACGAA TGACAGCACG CTAACGATAA 961 GAAAAGAAAT GCTATATGAT GTTGTAAACA ACATAAAAAA ATACAATGCC TACAGACATT 1021 AGTATAATTT CCTTTGATAT CAAAATGAAC CCTTTTATCC CTTACTTCGT TCGTTCATAG 1081 TTTCCATAAG AAACCGGAAC AGAGGAATTA AACTGC // |
| Primer: Amp_R | Sequence: RDB00919_A6Dmc.seq |
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>00919_00919_A6Dm_Amp_R_C09_09.ab1 1 ACTTTTCATA TTCAATCTTT TTGTTCCATT AAAGGGCGCT TAAAACCAGT CATACCAATA 61 ACTTAAGGGT AACTAGCCTC GCCGAAATGA TCGGCGATGA TGAAGTCTTT TTTTACCACT 121 TAATTTTAGA CGAAAAAGAA TATTTTTCGG GTCGTGAAAA ACTTGCAAAA GATGGCTATA 181 TCATGGATTC ATTAAATTTT ATAAATAGCC ATCAACCAAT TGAAATTTTT CATGATGGTA 241 GAATACATGT AATTCTAGTC ATTTAAAACG ATACATTAAT AGGTACGAAA AAGCAACTTT 301 TTTTGCGCCT AGAGGGCTTT TCGTGCGTCA GCATGAGCGA TCGGAAGAAA AGAAGAATAC 361 TTTTTCGCTT CAAGATGTTT TGCAGCGTGA TCGAGAACTT CGTGAGCAAA GAAAAGCAAA 421 GAGGAAAAAA TCGCATGATT TGGAGCGATA AGAAAAAGCA CTCGAATGAG TGCTTTTTTT 481 GCGTTTTGAG CGTAGCGAAA AACGAGTTCT TTCTATTCTT GATACATATA GAAATAACGT 541 CATTTTTATT TTAGTTGCTG AAAGGTGCGT TGAAGTGTTG GTATGTATGT GTTTTAAAGT 601 ATTGAAAACC CTTAAAATTG GTTGCACAGA AAAACCCCAT CTGTTAAAGT TATAAGTGAC 661 CAAACAAATA ACTAAATAGA TGGGGGTTTC TTTTAATATT ATGTGTCCTA ATAGTAGCAT 721 TTATTCAGAT GAAAAATCAA GGGTTTTAGT GGACAAGACA AAAAGTGGAA AAGTGAGACC 781 ATGGAGAGAA AAGAAAATCG CTAATGTTGA TTACTTTGAA CTTCTGCATA TTCTTGAATT 841 TAAAAAGGCT GAAAGAGTAA AAGATTGTGC TGAAATATTA GAGTATAAAC AAAATCGTGA 901 AACAGGCGAA AGAAAGTTGT ATCGAGTGTG GTTTTGTAAA TCCAGGCTTT GTCCAATGTG 961 CAACTGGGAG GAGAGCAATG AAACATGGCA TTCAGTCACA AAAGGTTGTT GCTGAAGTTA 1021 TTTAACAAAA GCCAACAGTT CGGTTGGGGT GGTTCTCACA TTAACAGTTA AAATGGTTAT 1081 GGATGGCCGA GATTAAAATA AAGAAGTTTT GTGTTTCCC // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Nakayama, N., A new shuttle vector for Bacillus stearothermophilus and Escherichia coli. Biotechnol. letters, 14, 649-652 (1992). |
|---|---|
| reference | Arutyunyan, A.M., Optical and magneto-optical activity of cytochrome bd from Geobacillus thermodenitrificans. Biochim. Biophys. Acta 1817 (11): 2087-2094 (2012). PMID 22728754. |
| reference | Noguchi, S., Over-expression of membrane-bound cytochrome c-551 from thermophilic Bacillus PS3 in Bacillus stearothermophilus K1041. Biochim. Biophys. Acta 1188 (3): 302-310 (1994). PMID 7803447. |
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl