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pSTE12 (#RDB00919)

Bacillus stearothermophilus shuttle vector

Clone info. Bacillus stearothermophilus shuttle vector
Vector backbone pSTE12 (Plasmid)
Size of vector backbone 5,800 bp
Selectable markers Amp^r (E. coli), Tet^r (B. stearothermophilus)
Growth remarks 55 degC for Bacillus stearothermophilus (LB+Tet).
Depositor|Developer Nakamoto, Shinya | Narumi, Issay |
 
Additional map
Remarks, protocol and/or map (pdf) RDB00919.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nakayama, N., Biotechnol. letters, 14, 649-652 (1992)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakayama, N., Biotechnol. letters, 14, 649-652 (1992))。

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RDB00919 pSTE12 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSTE12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB00919).

Reference section:

Nakayama, N., Narumi, I., Nakamoto, S., Kihara, H., A new shuttle vector for Bacillus stearothermophilus and Escherichia coli. Biotechnol. letters, 14, 649-652 (1992). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB00919_A6Dmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse
Sequence file: RDB00919_A6Dma.seq check
>00919_00919_A6Dm_SV40pA3ElementReverse_A09_03.ab1
    1 GTTTTGGGGG GGGCGGACCT ATGGAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
  121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
  181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
  241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG
  301 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA
  361 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA
  421 GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCGACCTGC
  481 AGGCATGCAA GCTTGGCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC
  541 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA
  601 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG
  661 ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC
  721 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT
  781 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC
  841 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG
  901 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG
  961 TCAAGTGGCA CTTTTTCGGG GGGAAAATGT GCGCGGGAAA CCCCCTATTG TTTAATTTTT
 1021 CTAAATACAT TCAAATAATG TATTCGCTTC ATGAGACAAT TAACCCTTGA TAATGGCTTT
1081 CAATAATAAT ACCTGGTATA AAAGGATCGA TTTGAAAC
//
Primer: pGEX-R
Sequence file: RDB00919_A6Dmb.seq check
>00919_00919_A6Dm_pGEX_R_B09_06.ab1
    1 TCATACGTAC GCGCGAGACG AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT
   61 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
  121 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
  181 ATAAATGCTT CAATAATAAT TCCTGTTATA AAAAAAGGAT CAATTTTGAA CTCTCTCCCA
  241 AAGTTGATCC CTTAACGATT TAGAAATCCC TTTGAGAATG TTTATATACA TTCAAGGTAA
  301 CCAGCCAACT AATGACAATG ATTCCTGAAA AAAGTAATAA CAAATTACTA TACAGATAAG
  361 TTGACTGATC AACTTCCATA GGTAACAACC TTTGATCAAG TAAGGGTATG GATAATAAAC
  421 CACCTACAAT TGCAATACCT GTTCCCTCTG ATAAAAAGCT GGTAAAGTTA AGCAAACTCA
  481 TTCCAGCACC AGCTTCCTGC TGTTTCAAGC TACTTGAAAC AATTGTTGAT ATAACTGTTT
  541 TGGTGAACGA AAGCCCACCT AAAACAAATA CGATTATAAT TGTCATGAAC CATGATGTTG
  601 TTTCTAAAAG AAAGGAAGCA GTTAAAAAGC TAACAGAAAG AAATGTAACT CCGATGTTTA
  661 ACACGTATAA AGGACCTCTT CTATCAACAA GTATCCCACC AATGTAGCCG AAAATAATGA
  721 CACTCATTGT TCCAGGGAAA ATAATTACAC TTCCGATTTC GGCAGTACTT AGCTGGTGAA
  781 CATCTTTCAT CATATAAGGA ACCATAGAGA CAAACCCTGC TACTGTTCCA AATATAATTC
  841 CCCCACAAAG AACTCCAATC ATAAAAGGTA TATTTTTCCC TAATCCGGGA TCAACAAAAG
  901 GATCTGTTAC TTTCCTGATA TGTTTTACAA ATATCACGAA TGACAGCACG CTAACGATAA
  961 GAAAAGAAAT GCTATATGAT GTTGTAAACA ACATAAAAAA ATACAATGCC TACAGACATT
 1021 AGTATAATTT CCTTTGATAT CAAAATGAAC CCTTTTATCC CTTACTTCGT TCGTTCATAG
1081 TTTCCATAAG AAACCGGAAC AGAGGAATTA AACTGC
//
Primer: Amp_R
Sequence file: RDB00919_A6Dmc.seq check
>00919_00919_A6Dm_Amp_R_C09_09.ab1
    1 ACTTTTCATA TTCAATCTTT TTGTTCCATT AAAGGGCGCT TAAAACCAGT CATACCAATA
   61 ACTTAAGGGT AACTAGCCTC GCCGAAATGA TCGGCGATGA TGAAGTCTTT TTTTACCACT
  121 TAATTTTAGA CGAAAAAGAA TATTTTTCGG GTCGTGAAAA ACTTGCAAAA GATGGCTATA
  181 TCATGGATTC ATTAAATTTT ATAAATAGCC ATCAACCAAT TGAAATTTTT CATGATGGTA
  241 GAATACATGT AATTCTAGTC ATTTAAAACG ATACATTAAT AGGTACGAAA AAGCAACTTT
  301 TTTTGCGCCT AGAGGGCTTT TCGTGCGTCA GCATGAGCGA TCGGAAGAAA AGAAGAATAC
  361 TTTTTCGCTT CAAGATGTTT TGCAGCGTGA TCGAGAACTT CGTGAGCAAA GAAAAGCAAA
  421 GAGGAAAAAA TCGCATGATT TGGAGCGATA AGAAAAAGCA CTCGAATGAG TGCTTTTTTT
  481 GCGTTTTGAG CGTAGCGAAA AACGAGTTCT TTCTATTCTT GATACATATA GAAATAACGT
  541 CATTTTTATT TTAGTTGCTG AAAGGTGCGT TGAAGTGTTG GTATGTATGT GTTTTAAAGT
  601 ATTGAAAACC CTTAAAATTG GTTGCACAGA AAAACCCCAT CTGTTAAAGT TATAAGTGAC
  661 CAAACAAATA ACTAAATAGA TGGGGGTTTC TTTTAATATT ATGTGTCCTA ATAGTAGCAT
  721 TTATTCAGAT GAAAAATCAA GGGTTTTAGT GGACAAGACA AAAAGTGGAA AAGTGAGACC
  781 ATGGAGAGAA AAGAAAATCG CTAATGTTGA TTACTTTGAA CTTCTGCATA TTCTTGAATT
  841 TAAAAAGGCT GAAAGAGTAA AAGATTGTGC TGAAATATTA GAGTATAAAC AAAATCGTGA
  901 AACAGGCGAA AGAAAGTTGT ATCGAGTGTG GTTTTGTAAA TCCAGGCTTT GTCCAATGTG
  961 CAACTGGGAG GAGAGCAATG AAACATGGCA TTCAGTCACA AAAGGTTGTT GCTGAAGTTA
 1021 TTTAACAAAA GCCAACAGTT CGGTTGGGGT GGTTCTCACA TTAACAGTTA AAATGGTTAT
1081 GGATGGCCGA GATTAAAATA AAGAAGTTTT GTGTTTCCC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakayama, N., A new shuttle vector for Bacillus stearothermophilus and Escherichia coli. Biotechnol. letters, 14, 649-652 (1992). [link to RRC of NBRP]
reference Arutyunyan, A.M., Optical and magneto-optical activity of cytochrome bd from Geobacillus thermodenitrificans. Biochim. Biophys. Acta 1817 (11): 2087-2094 (2012). PMID 22728754.
reference Noguchi, S., Over-expression of membrane-bound cytochrome c-551 from thermophilic Bacillus PS3 in Bacillus stearothermophilus K1041. Biochim. Biophys. Acta 1188 (3): 302-310 (1994). PMID 7803447.