RDB00919 - pSTE12

Resource data sheet
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pSTE12

Bacillus stearothermophilus shuttle vector

Catalog number	RDB00919
Resource name	pSTE12
Clone info.	Bacillus stearothermophilus shuttle vector
Vector backbone	pSTE12 (Plasmid)
Size of vector backbone	5,800 bp
Selectable markers	Amp^r (E. coli), Tet^r (B. stearothermophilus)
Gene/insert name	vector -
Depositor	Nakamoto, Shinya \| Narumi, Issay \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nakayama, N., Biotechnol. letters, 14, 649-652 (1992)).
(Japanese text [open/close])

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakayama, N., Biotechnol. letters, 14, 649-652 (1992))。

Catalog # Resource name Shipping form Fee (non-profit org.)

RDB00919 pSTE12 DNA solution

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nakayama, N., Biotechnol. letters, 14, 649-652 (1992)). (Japanese text [open/close]) 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakayama, N., Biotechnol. letters, 14, 649-652 (1992))。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB00919	pSTE12	DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB00919_A6Dmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse	RDB00919_A6Dma.seq
>00919_00919_A6Dm_SV40pA3ElementReverse_A09_03.ab1 1 GTTTTGGGGG GGGCGGACCT ATGGAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 301 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 361 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 421 GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCGACCTGC 481 AGGCATGCAA GCTTGGCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC 541 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA 601 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG 661 ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC 721 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT 781 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC 841 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG 901 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 961 TCAAGTGGCA CTTTTTCGGG GGGAAAATGT GCGCGGGAAA CCCCCTATTG TTTAATTTTT 1021 CTAAATACAT TCAAATAATG TATTCGCTTC ATGAGACAAT TAACCCTTGA TAATGGCTTT 1081 CAATAATAAT ACCTGGTATA AAAGGATCGA TTTGAAAC //
Primer: pGEX-R	RDB00919_A6Dmb.seq
>00919_00919_A6Dm_pGEX_R_B09_06.ab1 1 TCATACGTAC GCGCGAGACG AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT 61 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC 121 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG 181 ATAAATGCTT CAATAATAAT TCCTGTTATA AAAAAAGGAT CAATTTTGAA CTCTCTCCCA 241 AAGTTGATCC CTTAACGATT TAGAAATCCC TTTGAGAATG TTTATATACA TTCAAGGTAA 301 CCAGCCAACT AATGACAATG ATTCCTGAAA AAAGTAATAA CAAATTACTA TACAGATAAG 361 TTGACTGATC AACTTCCATA GGTAACAACC TTTGATCAAG TAAGGGTATG GATAATAAAC 421 CACCTACAAT TGCAATACCT GTTCCCTCTG ATAAAAAGCT GGTAAAGTTA AGCAAACTCA 481 TTCCAGCACC AGCTTCCTGC TGTTTCAAGC TACTTGAAAC AATTGTTGAT ATAACTGTTT 541 TGGTGAACGA AAGCCCACCT AAAACAAATA CGATTATAAT TGTCATGAAC CATGATGTTG 601 TTTCTAAAAG AAAGGAAGCA GTTAAAAAGC TAACAGAAAG AAATGTAACT CCGATGTTTA 661 ACACGTATAA AGGACCTCTT CTATCAACAA GTATCCCACC AATGTAGCCG AAAATAATGA 721 CACTCATTGT TCCAGGGAAA ATAATTACAC TTCCGATTTC GGCAGTACTT AGCTGGTGAA 781 CATCTTTCAT CATATAAGGA ACCATAGAGA CAAACCCTGC TACTGTTCCA AATATAATTC 841 CCCCACAAAG AACTCCAATC ATAAAAGGTA TATTTTTCCC TAATCCGGGA TCAACAAAAG 901 GATCTGTTAC TTTCCTGATA TGTTTTACAA ATATCACGAA TGACAGCACG CTAACGATAA 961 GAAAAGAAAT GCTATATGAT GTTGTAAACA ACATAAAAAA ATACAATGCC TACAGACATT 1021 AGTATAATTT CCTTTGATAT CAAAATGAAC CCTTTTATCC CTTACTTCGT TCGTTCATAG 1081 TTTCCATAAG AAACCGGAAC AGAGGAATTA AACTGC //
Primer: Amp_R	RDB00919_A6Dmc.seq
>00919_00919_A6Dm_Amp_R_C09_09.ab1 1 ACTTTTCATA TTCAATCTTT TTGTTCCATT AAAGGGCGCT TAAAACCAGT CATACCAATA 61 ACTTAAGGGT AACTAGCCTC GCCGAAATGA TCGGCGATGA TGAAGTCTTT TTTTACCACT 121 TAATTTTAGA CGAAAAAGAA TATTTTTCGG GTCGTGAAAA ACTTGCAAAA GATGGCTATA 181 TCATGGATTC ATTAAATTTT ATAAATAGCC ATCAACCAAT TGAAATTTTT CATGATGGTA 241 GAATACATGT AATTCTAGTC ATTTAAAACG ATACATTAAT AGGTACGAAA AAGCAACTTT 301 TTTTGCGCCT AGAGGGCTTT TCGTGCGTCA GCATGAGCGA TCGGAAGAAA AGAAGAATAC 361 TTTTTCGCTT CAAGATGTTT TGCAGCGTGA TCGAGAACTT CGTGAGCAAA GAAAAGCAAA 421 GAGGAAAAAA TCGCATGATT TGGAGCGATA AGAAAAAGCA CTCGAATGAG TGCTTTTTTT 481 GCGTTTTGAG CGTAGCGAAA AACGAGTTCT TTCTATTCTT GATACATATA GAAATAACGT 541 CATTTTTATT TTAGTTGCTG AAAGGTGCGT TGAAGTGTTG GTATGTATGT GTTTTAAAGT 601 ATTGAAAACC CTTAAAATTG GTTGCACAGA AAAACCCCAT CTGTTAAAGT TATAAGTGAC 661 CAAACAAATA ACTAAATAGA TGGGGGTTTC TTTTAATATT ATGTGTCCTA ATAGTAGCAT 721 TTATTCAGAT GAAAAATCAA GGGTTTTAGT GGACAAGACA AAAAGTGGAA AAGTGAGACC 781 ATGGAGAGAA AAGAAAATCG CTAATGTTGA TTACTTTGAA CTTCTGCATA TTCTTGAATT 841 TAAAAAGGCT GAAAGAGTAA AAGATTGTGC TGAAATATTA GAGTATAAAC AAAATCGTGA 901 AACAGGCGAA AGAAAGTTGT ATCGAGTGTG GTTTTGTAAA TCCAGGCTTT GTCCAATGTG 961 CAACTGGGAG GAGAGCAATG AAACATGGCA TTCAGTCACA AAAGGTTGTT GCTGAAGTTA 1021 TTTAACAAAA GCCAACAGTT CGGTTGGGGT GGTTCTCACA TTAACAGTTA AAATGGTTAT 1081 GGATGGCCGA GATTAAAATA AAGAAGTTTT GTGTTTCCC //

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

Electronic file	Map RDB00919.gif from Depositor
Electronic file	PDF RDB00919.pdf from Depositor

Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

original	Nakayama, N., A new shuttle vector for Bacillus stearothermophilus and Escherichia coli. Biotechnol. letters, 14, 649-652 (1992).
reference	Noguchi, S., Over-expression of membrane-bound cytochrome c-551 from thermophilic Bacillus PS3 in Bacillus stearothermophilus K1041. Biochim. Biophys. Acta 1188 (3): 302-310 (1994). PMID 7803447.