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pAxc

A cassette cosmid for construction of recombinant adenovirus

Catalog number RDB00918
Resource name pAxc
Alternative name pAdex1c
Clone info. A cassette cosmid for construction of recombinant adenovirus.
Produce adenovirus by COS-TPC method.
Vector backbone pAxc (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42,464 bp
Selectable markers Amp^r
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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Remarks Remember that you will be working with samples containing infectious virus.
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RDB00918 pAxc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

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References

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

2018.12.10

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