JCM20135 Genomic DNA

Resource data sheet
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Genomic DNA of "Escherichia coli" (#JGD07547)

Catalog Number	JGD07547
Source strain	JCM 20135 [link]

Present DNA Lot	14292_B3Gc
Shipping size	Approx. 71 ng/ul, 50 ul/vial
Remarks

The authenticity of microbial genomic DNAs for the distribution are confirmed by PCR amplification of 16S rRNA gene and nucleotide sequencing of the amplified products. However, these tests do not exclude contamination of any other species. Please be aware that the genomic DNA send from us may not necessarily fit for your specific research purpose.
If you have any questions, please feel free to contact us.

Distribution information

Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Material Transfer Agreement (MTA, for use for for-profit purpose)[Word] Please put "Terms and conditions" to the Clause 4 of the MTA.
Terms and conditions	The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.

提供案内 (日本国内)

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提供依頼	手続きの詳細は、「BRC-JCM保有株由来ゲノムDNA」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利学術目的用)[Word] 提供同意書 (営利目的用)[Word] MTAの第４項目には「使用条件」を転記してください。
MTAに書く使用条件	本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。

Catalog #	Resource name	Shipping form	Fee
JGD07547	Genomic DNA of Escherichia coli JCM 20135	DNA

How to cite this biological resource

Materials & Methods section:

The Genomic DNA of Escherichia coli JCM 20135 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. JGD07547).

References and tips

Microbe Genomic DNA Information [link]

Reconstitution of DNA (in the case you received plastic vials contains vacuum dried DNA).
Primers for PCR of rRNA gene.

Featured content

Featured content	Dnaconda's recommendation EXR0018j (Japanese text)

References

user_report - reference	Ikei, M., YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition. PLoS Biol. 22 (4): e3002601 (2024). PMID 38656967. [PubMed] [Article] [RRC of NBRP]
user_report - reference	Tanaka, Y., Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake. Sci. Adv. 6(35): eaba7637 (2020). PMID 32923628. [PubMed] [Article] [RRC of NBRP]
user_report - reference	Tsuchiya, H., Structural basis for amino acid export by DMT superfamily transporter YddG. Nature 534 (7607): 417-420 (2016). PMID 27281193. [PubMed] [Article] [RRC of NBRP]
user_report - reference	Lee, Y., Structural basis for the facilitative diffusion mechanism by SemiSWEET transporter. Nat. Commun. 6: 6112 (2015). PMID 25598322. [PubMed] [Article] [RRC of NBRP]
user_report - reference	Fukuda, M., Structural basis for dynamic mechanism of nitrate/nitrite antiport by NarK. Nat. Commun. 6: 7097 (2015). PMID 25959928. [PubMed] [Article] [RRC of NBRP]
user_report - reference	Kobayashi, K., Crystallization and preliminary X-ray crystallographic analysis of YfcM: an important factor for EF-P hydroxylation. Acta Crystallogr. F Struct. Biol. Commun. 70 (Pt 9): 1236-1239 (2014). PMID 25195899. [PubMed] [Article] [RRC of NBRP]

2025.04.10

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