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Empty vectors for construction of recombinant adenovirus

Catalog num. Clone name
Description
Cloning sitePromoteraCre-loxPbProduction of recombinant adenovirus
Intact-genome transfectioncTwo-cosmiddCOS-TPCeFiber-modified
COS-TPC
RDB00917 pAxcw
A cassette cosmid for construction of recombinant adenovirus
SwaI    Yes 
RDB01678 pAxCAwt
A cassette cosmid for construction of a recombinant adenovirus
SwaICAG   Yes 
RDB02565 pAxCEAwt
Cloning vector to generate recombinant adenovirus driven by human CEA promoter
SwaICEA   Yes 
RDB03120 pAxcwit
A dual cassette for constructing recombinant adenovirus
SwaI  Yes Yes 
RDB03121 pAxCAwtit
A dual cassette for constructing recombinant adenovirus containing CAG promoter
SwaICAG Yes Yes 
RDB03444 pUAd5L
Shuttle vector to generate recombinant adenovirus
SwaI   YesYesYes
RDB03445 pUAd5LCA
Shuttle vector to generate recombinant adenovirus
SwaICAG  YesYesYes
RDB03446 pUAd5LCALNL
Shuttle vector to generate recombinant adenovirus
SwaICAGYes YesYesYes
RDB05212 pAxcwit2
A dual cassette for constructing recombinant adenovirus
SwaI  Yesg Yes 
RDB05213 pAxCAwtit2
A dual cassette for constructing recombinant adenovirus
SwaICAG Yesg Yes 
RDB05214 pAxCALNLwtit2
A dual cassette for constructing recombinant adenovirus
SwaICAGYesYesg Yes 
RDB05215 pAxEFwtit2
A dual cassette for constructing recombinant adenovirus
SwaIEF-1 alpha Yesg Yes 
RDB06016 pAdex CAG-SwaI-IRES-EGFP
Shuttle vector to generate rAd with CAG expression unit. EGFP marker.
SwaICAG   Yes 
RDB00918 pAxc
A cassette cosmid for construction of recombinant adenovirus
RDB02564 pAxCEAwtR
Cloning vector to generate recombinant adenovirus driven by human CEA promoter
RDB03220 pAF16Rct
Cosmid clone of genomic fragment of adenovirus type 5
RDB03380 pAxCAiLacZit
Shuttle vector to generate recombinant adenovirus harbouring E. coli LacZ


a, CA, a modified promoter of the chicken gene for beta-actin with a cytomegalovirus-immediate early enhancer (CMV-IE); CEA, carcinoembryonic antigen; EF-1 alpha, elongation factor 1 alpha.

b, Target gene harbored in the recombinant adenovirus can be expressed by co-infection with AxCANCre (RDB no. 1748, Gene 181: 207-212, 1996).

c, Fukuda et al., Microbiol. Immunol. 50: 643-654, 2006.

d, Use pAF16Rct (RDB no. 3220) together to generate rAd.

e, Miyake et al., Proc. Natl. Acad. Sci. USA 93: 1320-1324, 1996.

g, PacI restriction enzyme is also applicable for linearization as well as Csp45I restriction enzyme.

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(2010.05.19 T.M.)

2018.05.04